Journal: Diabetology & Metabolic Syndrome
Article Title: Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus
doi: 10.1186/s13098-025-01852-z
Figure Lengend Snippet: Pyroptosis induced by NETs was regulated by NLRP1. A . Western blotting was performed to detect the expression of related proteins in pyroptosis in Min6 cells with NLRP1 knocked down; B . ELISA was used to detect the LDH, IL-1βand IL-18; C - D . Flow cytometry was used to detect pyroptosis in NLRP1 knocked down Min6 cells induced by NETs; N = 3, * P < 0.05, ** P < 0.01
Article Snippet: Mouse Irisin ELISA kit (E-EL-M2743), LDH ELISA kit (E-EL-M0419), IL-18 ELISA kit (E-EL-M0730) were purchased from Elabscience.
Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry